Jove
Visualize
Contact Us

Related Experiment Videos

Picoliter (1)H NMR spectroscopy.

Kevin R Minard1, Robert A Wind

  • 1Pacific Northwest National Laboratory, Richland, WA 99352, USA.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|February 16, 2002
PubMed
Summary

This study demonstrates the feasibility of performing proton nuclear magnetic resonance ((1)H NMR) on picoliter-scale biological samples. Researchers achieved high signal-to-noise ratios (SNR) in microscopic volumes, enabling detailed cellular analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Risks of Aldehyde Constituents in Cigarette Smoke Using Transient Computational Fluid Dynamics/Physiologically Based Pharmacokinetic Models of the Rat and Human Respiratory Tracts.

Toxicological sciences : an official journal of the Society of Toxicology·2015
Same author

Comparative iron oxide nanoparticle cellular dosimetry and response in mice by the inhalation and liquid cell culture exposure routes.

Particle and fibre toxicology·2014
Same author

Slow-MAS NMR: A new technology for in vivo metabolomic studies.

Drug discovery today. Technologies·2014
Same author

Iron oxide nanoparticle agglomeration influences dose rates and modulates oxidative stress-mediated dose-response profiles in vitro.

Nanotoxicology·2013
Same author

Magnetic particle detection (MPD) for in-vitro dosimetry.

Biosensors & bioelectronics·2013
Same author

Phase-contrast MRI and CFD modeling of apparent ³He gas flow in rat pulmonary airways.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2012
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Biophysics
  • Cellular Biology
  • Spectroscopy

Background:

  • Nuclear Magnetic Resonance (NMR) is a powerful technique for molecular analysis.
  • Achieving high signal-to-noise ratios (SNR) in small biological samples remains a challenge for NMR.
  • Microscopic NMR requires specialized hardware to overcome sensitivity limitations.

Purpose of the Study:

  • To demonstrate the feasibility of (1)H NMR spectroscopy on picoliter-scale biological samples.
  • To evaluate the performance of a solenoid transceiver for localized NMR.
  • To validate SNR prediction models for microscopic spatial resolutions.

Main Methods:

  • Utilized a 267-microm-diameter solenoid transceiver for localized (1)H NMR.
  • Acquired spectra from micro-samples: triacylglycerol globule (100-microm diameter) and choline solution (33 mM).
  • Performed chemical shift imaging (CSI) experiments with thousands of scans.

Main Results:

  • Measured SNR approached theoretical limits (20-30% of limits considering resistive losses).
  • Achieved acceptable SNR for choline methyl line in 50 pL sample volumes.
  • Resolved localized spectra from as little as 5 pL in Xenopus samples.

Conclusions:

  • (1)H NMR is feasible for picoliter-scale sample volumes in biological cells and tissues.
  • The developed method enables high-resolution spectroscopic imaging.
  • SNR in spectroscopic images can be accurately predicted at microscopic resolutions.

Related Experiment Videos