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[Mutation BRCA1 gene in 186 breast cancer patients]
1Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology]
|February 28, 2002
Abstract
Objective:
To analysis the mutation of BRCA1 gene in 186 breast cancer patients.
Methods:
Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and sequential were used in this analysis.
Results:
13/186 of patients showed mutation which comprised 7% of the total number of patients. In 8 patients, the mutation was observed in the intron splicing region and in 5 patients, the mutation was on the exon.
Conclusion:
Mutation analysis of BRCA1 gene is helpful in the determination of developmental potential, early diagnosis and gene therapy for breast cancer.