Posterior thigh perforator-based flap: a new experimental model in rats

Area of Science:

• Surgical Innovation
• Animal Models in Research
• Flap Physiology

Background:

• Animal research is crucial for understanding flap hemodynamics.
• Rats are frequently used in flap research, necessitating refined models.
• Existing flap models vary, prompting the development of new approaches.

Purpose of the Study:

• To develop and validate a novel single-perforator-based flap model in rats.
• To assess the survival patterns of this new flap model with varying dimensions.
• To provide a reliable tool for investigating perforator-based flap physiology.

Main Methods:

• Anatomic dissection of the posterior thigh in rats to identify perforator arteries.
• Elevation of a single musculocutaneous perforator flap based on the biceps femoris artery.
• Evaluation of flap survival in three groups with different flap dimensions (3x2 cm, 3x4 cm, 3x12 cm).
• Microangiography to visualize the vascular architecture of the flap pedicle.

Main Results:

• The new flap model, based on a single perforator artery from the biceps femoris muscle, was successfully developed.
• Flaps with dimensions of 3x2 cm and 3x4 cm demonstrated 100% survival at one week.
• An oversized flap (3x12 cm) showed a survival rate of 61 +/- 7.2% at one week.
• Microangiography confirmed the vascular supply via the perforator artery.

Conclusions:

• The developed posterior thigh perforator-based flap model is simple, reliable, and exhibits consistent survival.
• This model is suitable for future research on the physiological and pathophysiological aspects of perforator flaps.
• The study provides a valuable new tool for the field of reconstructive surgery research.