[The influence of pressure on human lamina cribrosa cell and its collagen synthesis]
Related Experiment Video
Updated: Oct 2, 2026
Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
Published on: April 22, 2019
1Department of Ophthalmology, Union Hospital, Tong ji Medical University, Wuhan 430022.
Objective:
To study the influence of pressure on human lamina cribrosa cell (HLC) and its synthesis of collagen.
Method:
HLCs were cultured in vitro and submitted to elevated hydrostatic pressure, their changes in morphology were observed by light and electron microscopy and their synthesis of collagen was measured with fluorescence spectrophotometer.
Results:
Under a hydrostatic pressure of 6.67 kPa (1 kPa = 7.5 mmHg), the flat and polygonal HLCs became elongated. Vacuoles appeared in the cytoplasm and increased as the pressure persisted. The mitochondria were swollen, some of them turned into vacuoles, and myelin figures were found in the cytoplasm. When exposed to elevated pressure for 3 days, the amounts of synthesized collagen type I, III, IV were 43.95 +/- 6.37, 61.35 +/- 10.35 and 82.90 +/- 11.36 arbitrary unit (AU) by fluorometry respectively, and compared to controls, they were statistically significantly (P < 0.05) increased; after 5 days of pressure exposure, the amounts were 33.50 +/- 6.94, 40.85 +/- 12.30 and 80.45 +/- 14.65 AU, e.g., still increased.
Conclusion:
It is indicated that under pressure, HLCs undergo obvious morphological changes and increase their synthesis of collagen types I, III and IV.
Related Concept Videos
01:13Cell-matrix's Response to Mechanical Forces
Anchoring junctions mechanically attach a cell to the...
00:55Laminins are the Adhesive Proteins of Basal Lamina
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...