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Quantification of ephedrines in urine by column-switching high-performance liquid chromatography
G Gmeiner1, T Geisendorfer, J Kainzbauer
1Chemical Analysis, Austrian Research Center Seibersdorf, Austria. guenter.gmeiner@arcs.ac.at
Abstract:
A method for the quantification of five congener ephedrines in urine samples without sample preparation was developed. The analytes were trapped on a C18 precolumn and separated on a C18 BDS analytical column. Baseline separation was achieved for all analytes. The method meets the requirements of the International Olympic Committee (IOC) medical commission regarding cut-off limits for positive doping cases with ephedrines.