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Updated: Jul 13, 2026

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
Published on: September 18, 2019
Intramolecular Förster energy transfer in a dendritic system at the single molecule level
Roel Gronheid1, Johan Hofkens, Fabian Köhn
1Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium.
Abstract:
The photophysics of a dendrimer containing four donor chromophores and one acceptor chromophore are studied at the single-molecule level. Upon excitation of the donors exclusive acceptor emission is observed due to efficient Förster energy transfer. For 70% of the molecules donor emission is observed after bleaching of the acceptor, leading to a reduction of the Förster energy transfer efficiency. Furthermore, we demonstrate that in this molecular system the donor chromophores do not bleach by a triplet-sensitized photooxidation.
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