[Method for the primary selection of active antitumor alkylating preparations in vitro and in vivo]

To reveal the cytotoxic activity of antitumor drugs the experiments were conducted in vitro-in vivo. Active substances in the dosage of 800 mg per 1 Kg of tumor were found to suppress sarcoma 45 transplantability during 1 hour in vitro. But inactive substances did it only in much larger doses. The result of the in vivo-in vitro experiments coincided with the results obtained by other investigators studying the antitumor activity in vivo. The experiments using the proposed method of selection required a much less amount of the substance (a biological experiment, repeated twice, can be carried out having 1--3 mg), animals, time and efforts of the researcher.