Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cellular platforms for HTS: three case studies.

Paul A Johnston1, Patricia A Johnston

  • 1Sphinx Laboratories, Lilly Research Laboratories, A Division of Eli Lilly and Company, 20 T.W. Alexander Drive, PO Box 13951, Research Triangle Park, NC 27709, USA.

Drug Discovery Today
|March 15, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimization and calibration of 384-well kinetic Ca<sup>2+</sup> mobilization assays for the human transient receptor potential cation channels TRPM8, TRPV1, and TRPA1.

SLAS discovery : advancing life sciences R & D·2025
Same author

Miniaturization and characterization of patient derived hepatocellular carcinoma tumor organoid cultures for cancer drug discovery applications.

SLAS discovery : advancing life sciences R & D·2024
Same author

Discovery of Benzisothiazolone Derivatives as Bifunctional Inhibitors of HIV-1 Reverse Transcriptase DNA Polymerase and Ribonuclease H Activities.

Biomolecules·2024
Same author

Evaluating Readmission Rates for a Statewide In-Home Ecosystemic Family-Based Treatment Program for Youth with Serious Emotional Disturbance.

Community mental health journal·2024
Same author

Subcutaneous administration of a novel TRPM8 antagonist reverses cold hypersensitivity while attenuating the drop in core body temperature.

British journal of pharmacology·2024
Same author

Replacement of the hydroxamic acid group in the selective HDAC8 inhibitor PCI-34051.

Bioorganic & medicinal chemistry letters·2024
Same journal

Targeting the GLP-1 receptor pathways for dual management of obesity and depression.

Drug discovery today·2026
Same journal

Chemical intervention strategies targeting MYC for cancer therapy.

Drug discovery today·2026
Same journal

How many protein pairs can we chemically target?

Drug discovery today·2026
Same journal

From trial-and-error to inverse design: how AI is redefining drug delivery systems.

Drug discovery today·2026
Same journal

Critical evaluation of the key mediators causing life-threatening symptoms during human anaphylaxis.

Drug discovery today·2026
Same journal

A20 as a novel immunoregulatory target for neuroinflammation.

Drug discovery today·2026
See all related articles

Cell-based screening offers rich data on compound activity but presents implementation challenges. This review details successful cell-based high-throughput screening (HTS) case studies and validation strategies.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Drug Discovery

Background:

  • Cell-based screening is rapidly advancing due to innovations in target selection and instrumentation.
  • Cell-based assays provide comprehensive data on chemical compound activity, mechanism of action, and drugability.
  • Implementing cell-based assays in high-throughput screening (HTS) laboratories can be challenging.

Purpose of the Study:

  • To review the successful application of cell-based high-throughput screening (HTS) for target interrogation.
  • To highlight key considerations and necessary steps for validating cell-based HTS assays.
  • To provide insights into overcoming challenges in cell-based screening implementation.

Main Methods:

  • Review of three case studies involving cell-based high-throughput screening (HTS).

Related Experiment Videos

  • Analysis of target interrogation strategies within cellular formats.
  • Identification of critical steps for ensuring assay validity and reliability.

    • Main Results:

    • Successful interrogation of targets using cell-based HTS was demonstrated in the presented case studies.
    • Key factors contributing to the success of cell-based HTS assays were identified.
    • Strategies for validating cell-based screens were highlighted, ensuring data integrity.

    Conclusions:

    • Cell-based screening is a powerful tool for drug discovery, offering detailed biological insights.
    • Careful planning and validation are crucial for successful implementation of cell-based HTS.
    • Overcoming implementation challenges enables efficient and reliable target interrogation in cellular assays.