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Updated: Sep 29, 2026

Detection of Histone Modifications in Plant Leaves
Published on: September 23, 2011
Arabidopsis glutathione-dependent formaldehyde dehydrogenase is an S-nitrosoglutathione reductase
Atsushi Sakamoto1, Manami Ueda, Hiromichi Morikawa
1Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, 739-8526, Higashi-Hiroshima, Japan. ahkkao@hiroshima-u.ac.jp
Abstract:
S-Nitrosoglutathione (GSNO), an adduct of nitric oxide (NO) with glutathione, is known as a biological NO reservoir. Heterologous expression in Escherichia coli of a cDNA encoding a glutathione-dependent formaldehyde dehydrogenase of Arabidopsis thaliana showed that the recombinant protein reduces GSNO. The identity of the cDNA was further confirmed by functional complementation of the hypersensitivity to GSNO of a yeast mutant with impaired GSNO metabolism. This is the first demonstration of a plant GSNO reductase, suggesting that plants possess the enzymatic pathway that modulates the bioactivity and toxicity of NO.
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