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Updated: Oct 1, 2026

Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice
Published on: March 23, 2014
[Effect of various storage methods on the viability of myxobacteria]
1I.I. Mechnikov Odessa State University, Department of Microbiology and Virology, 2 Dvoryanskaya St., Odessa, 65026, Ukraine.
Abstract:
Viability of myxobacteria strains Myxococcus xanthus UNCM 10041, Polyangium cellulosum UNCM 10043, Archangium gephyra UNCM 10001 stored by the methods of lyophilization, cryoconservation, drying on paper discs, suspending in distilled water or physiological solution under a layer of mineral oil. The best results on viability preservation of all three studied strains were obtained when using the method of cryoconservation. It has been shown that the strains M. xanthus and A. gephyra preserve the best viability during two years of observations when stored in liquid nitrogen and dried on paper discs. Strain P. cellulosum preserves viability for two years when stored in liquid nitrogen and in suspensions on distilled water and physiological solution.
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