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Mouse Models Of Helicobacter Infection And Gastric Pathologies
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[Invasiveness and cytopathogenicity of Helicobacter pylori in human cell culture]

O P Konareva1, V A Ivanitsa

  • 1Odessa National University, Department of Microbiology and Virology, Dvoryanskaya St., Odessa, 65026, Ukraine.

Mikrobiolohichnyi Zhurnal (Kiev, Ukraine : 1993)
|November 19, 2003
PubMed

Insights

Helicobacter pylori exhibits strong invasion and adhesion, disrupting cell division and causing DNA damage in Hep-2 cell cultures. This bacterium promotes neoplastic growth, indicating a potential role in cancer development.

Area of Science:

  • Microbiology
  • Cell Biology
  • Oncology

Background:

  • Helicobacter pylori is a common human pathogen linked to gastric diseases.
  • Its pathogenic mechanisms, particularly cellular effects, require further elucidation.

Purpose of the Study:

  • To investigate the invasive and cytopathogenic effects of clinical Helicobacter pylori strains on Hep-2 cells.
  • To assess the impact of H. pylori on cellular proliferation and genetic stability.

Main Methods:

  • Co-cultivation of clinical Helicobacter pylori strains with Hep-2 cell lines.
  • Microscopic examination for invasion and cytopathic effects.
  • Analysis of mitotic activity, including mitotic index and chromosomal aberrations.
  • Observation of neoplastic transformation indicators like loss of contact inhibition.

Main Results:

  • Helicobacter pylori demonstrated significant invasive and adhesive capabilities in Hep-2 cells.
  • The bacterium markedly affected mitotic activity, altering the mitotic index and increasing pathogenic mitoses.
  • H. pylori induced pathologies related to mitotic apparatus disturbance and chromosomal damage.
  • Cells exposed to H. pylori showed a propensity for neoplastic growth, including loss of contact inhibition and formation of transformation foci.

Conclusions:

  • Clinical strains of Helicobacter pylori possess potent invasive and cytopathogenic properties.
  • H. pylori significantly disrupts host cell division and induces genetic instability.
  • The observed neoplastic changes in Hep-2 cells suggest a potential oncogenic role for H. pylori.

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