Related Experiment Video
Updated: Oct 1, 2026
In vitro Uncoating of HIV-1 Cores
Published on: November 8, 2011
[Isolation of HIV-1 from infected individuals in Fujian Province]
Objective:
To isolate the virus from HIV-1 infected individuals.
Methods:
Blood specimens were collected from 10 HIV-1 infected persons in Fujian Province. Peripheral blood mononuclear cells (PBMCs) were separated from the heparinized blood. Each sample was cocultured with fresh normal PBMCs to isolate HIV-1. Supernatant of the cocultures was tested by using HIV-1 P24 antigen Kit.
Results:
Of the 10 specimens, 9 showed positive for the P24. 4 of 9 isolated infected MT4 cells, and cell syncytias could be seen. Three isolates showed transient infection of MT4, which probably belong to slow/low virus. Only one isolate could infect MT4 successively, which had been passed for 15 generations without significantly changed viral titer; it is believed to be a rapid/high replication virus.
Conclusions:
HIV-1 virus was successfully isolated.
