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[Expression of baculovirus recombinant HPV16L1 and L2 capsid proteins in insect cells]
1Institute of Virology, Chinese Academy of Preventive Medicine,Beijing 100052, China.
Background:
To express capsid proteins of HPV16 in insect cells system.
Methods:
Recombinant baculovirus stocks were generated by cotransfection with baculovirus DNA and baculovirus recombinant transfer plasmids. The expression of aimed proteins in insect cells were confirmed by using SDS-PAGE and Western blot.
Results:
Two stable strains of recombinant baculovirus expressing HPV16L1 protein alone and co-expressing HPV16L1 plus L2 proteins were obtained. The L1 and L2 ORFs of HPV16 produced proteins of 57000 and 97000, respectively. The yield of L1 protein was about 25%-30% of the total proteins of the insect cell.
Conclusions:
L1 and L2 proteins of HPV16 could be expressed with high efficiency in insect cells via recombinant baculoviruses.
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