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[Malignant transformation of monkey kidney epithelial cell induced by EBV BARF1 gene and TPA]
1Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Background:
To study the effect of Epstein-Barr virus (EBV) BARF1 gene alone and synergistic effect of the gene and TPA in carcinogenesis.
Methods:
PT1 and PT7 cell line which were immortalized monkey kidney epithelial cells induced by the BARF1 gene of EB virus.PT1 and PT7 cell were cultured with 5 ng/ml TPA for two weeks. Then the nude mice and scid mice were injected with PT1, PT7, PT1+TPA, PT7+TPA cells, respectively.
Results:
The immortalized PT1 and PT7 cells could not develop the tumor in nude mice, but could develop the tumor in scid mice (severe combined immune deficiency mice). It took about 45-60 days for development of tumor. After adding the tumor promoter TPA, the PT1 and PT7 cells developed the tumor either in nude mice or scid mice. It needed about 20-22 days in nude mice and 5-7 days in scid mice for development of tumor. PCR amplification revealed EBV-BARF1 gene in tumor tissues.
Conclusions:
EB virus BARF1 gene is another oncogene. Malignant transformation of the primary monkey kidney epithelial cell can be induced with BARF1 gene,even without tumor promoter.
