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Effects of lactated Ringer's solutions on human leukocytes
Elena Koustova1, Kathleen Stanton, Vadim Gushchin
1Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA. ekoustova@ushs.mil
The Journal of Trauma
|May 4, 2002
Summary
Standard lactated Ringer's (LR) solution, a racemic mixture, significantly impacts neutrophil function and leukocyte gene expression. This racemic mixture (DL-LR) increases reactive oxygen species production compared to L-LR.
Area of Science:
- Immunology
- Cell Biology
- Pharmacology
Background:
- Standard lactated Ringer's (LR) solution utilizes a racemic mixture of lactate isomers (D- and L-).
- The differential effects of racemic lactate Ringer's (DL-LR) versus L-isomer only (L-LR) on immune cells remain largely uncharacterized.
Purpose of the Study:
- To investigate the distinct effects of DL-LR and L-LR on neutrophil function and leukocyte gene expression.
- To compare these effects against other intravenous solutions like Hank's balanced salt solution, normal saline, and ketone Ringer's.
Main Methods:
- Incubation of volunteer blood with various Ringer's lactate formulations and control solutions.
- Measurement of neutrophil oxidative burst activity via flow cytometry.
- Analysis of gene expression for 23 leukocyte-associated genes using cDNA array technology.
Main Results:
- DL-LR significantly increased reactive oxygen species production by neutrophils compared to L-LR and ketone Ringer's.
- DL-LR altered the expression of leukocyte genes involved in inflammatory responses, cell migration, and apoptosis.
- L-LR and ketone Ringer's demonstrated less pronounced effects on these cellular functions.
Conclusions:
- The commonly used racemic formulation of lactated Ringer's (DL-LR) exerts a notable influence on neutrophil function.
- DL-LR impacts leukocyte gene expression profiles, suggesting a role in modulating inflammatory and apoptotic pathways.