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An infallible method for producing ascitic fluid from solid tumours.

R F Altman, A R Mendes, P M Cotias

    Archiv Fur Geschwulstforschung
    |January 1, 1975
    PubMed
    Summary
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    Surfactants significantly improve the transformation of solid tumors into ascites form by reducing cell adhesion. This method shows promise for ascites production in cancer research, overcoming previous limitations.

    Area of Science:

    • Oncology
    • Biochemistry
    • Cell Biology

    Background:

    • Solid tumors often resist transformation into ascites form, hindering research.
    • High intercellular adhesion forces in tumors are a key challenge.
    • Cancer cells typically have a higher negative electrical charge, affecting adhesiveness.

    Purpose of the Study:

    • To investigate the influence of anionic, nonionic, and amphoteric detergents on ascites fluid production from tumor macerates.
    • To overcome the difficulty in producing ascites form from fibrosarcoma BUSP.
    • To evaluate the efficacy of surfactants in facilitating tumor transformation.

    Main Methods:

    • Comparative experiments using Yoshida sarcoma and fibrosarcoma BUSP macerates.
    • Application of anionic (heparin), nonionic (Pluronic F-68), and amphoteric (phosphatidylcholine) detergents at various concentrations.

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  • Control groups without detergent treatment.

    • Main Results:

    • Detergent treatment resulted in successful ascites fluid production in all treated animals, unlike controls which showed high failure rates (up to 100% for BUSP).
    • Surfactants effectively reduced intercellular adhesion, promoting ascites formation.
    • High concentrations of heparin (1 mg/ml) caused tumor cell lysis, inhibiting ascites formation.

    Conclusions:

    • Anionic, nonionic, and amphoteric surfactants are highly effective in promoting ascites fluid production from solid tumors.
    • Surfactant-mediated reduction of cell adhesion is crucial for successful ascites transformation.
    • Careful selection of detergent type and concentration is necessary to avoid tumor cell lysis.