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Supercoils in human DNA.

P R Cook, I A Brazell

    Journal of Cell Science
    |November 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Human DNA exhibits supercoiling, a structural property influencing its behavior. Intercalating agents reveal this supercoiling in nuclear structures, a change reversed by gamma irradiation and heat treatment.

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    Area of Science:

    • Molecular Biology
    • Biophysics

    Background:

    • The three-dimensional structure of DNA involves helical and superhelical turns.
    • Superhelical DNA exhibits distinct hydrodynamic properties upon interaction with intercalating agents.

    Purpose of the Study:

    • To investigate the presence and characteristics of supercoiling in human nuclear DNA.
    • To explore the influence of intercalating agents and gamma irradiation on human DNA structure.

    Main Methods:

    • Analysis of hydrodynamic properties of nuclear structures from human cells.
    • Treatment with intercalating agents.
    • Irradiation of cells with gamma-rays and subsequent incubation at 37°C.

    Main Results:

    • Intercalating agents induced characteristic changes in the hydrodynamic properties of human nuclear structures.

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  • Gamma irradiation abolished these characteristic changes.
  • Incubation at 37°C post-irradiation restored the characteristic changes.

    • Conclusions:

    • Human DNA possesses supercoiled structures.
    • These findings suggest a model for chromosome structure involving supercoiled DNA.