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A sensitive and quantitative element-tagged immunoassay with ICPMS detection.
Vladimir I Baranov1, Zoë Quinn, Dmitry R Bandura
1MDS-SCIEX, Concord, Ontario, Canada.
Analytical Chemistry
|May 30, 2002
Summary
We developed new immunoassays using element-tagged antibodies and ICP-MS for sensitive protein detection. This method accurately quantifies proteins at low concentrations, offering a wide linear range for various applications.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Immunology
Background:
- Immunoassays are crucial for detecting biomolecules.
- Quantifying low protein concentrations requires sensitive and accurate methods.
- Elemental detection offers a novel approach to immunoassay signal amplification.
Purpose of the Study:
- To develop and validate novel immunoassays for protein detection.
- To couple immunoassays with Inductively Coupled Plasma Mass Spectrometry (ICP-MS) for elemental quantification.
- To assess the sensitivity and dynamic range of the developed immunoassay.
Main Methods:
- Utilized element-tagged antibodies for specific protein binding.
- Directly coupled immunoassays with ICP-MS for elemental (metal) detection.
- Validated protein detection using standard protein solutions.
Main Results:
- Achieved detection limits for target proteins as low as 0.1-0.5 ng/mL.
- Demonstrated a linear response of the assay across three orders of magnitude of protein concentration.
- Successfully quantified elemental tags corresponding to bound antibodies.
Conclusions:
- The developed element-tagged immunoassays coupled with ICP-MS provide a highly sensitive and linear method for protein quantification.
- This approach offers a robust alternative to traditional immunoassay detection methods.
- The technique has potential applications in diagnostics and biomarker discovery.