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The bacteriorhodopsin gene.

R Dunn1, J McCoy, M Simsek

  • 1Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.

Proceedings of the National Academy of Sciences of the United States of America
|November 1, 1981
PubMed
Summary
This summary is machine-generated.

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Researchers identified the bacteriorhodopsin gene in Halobacterium halobium DNA. The gene sequence reveals the standard genetic code is used, with specific codon preferences and no intervening sequences.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Bacteriorhodopsin is a light-driven proton pump found in Halobacterium halobium.
  • Understanding its gene structure provides insights into archaebacterial gene expression.

Purpose of the Study:

  • To identify and characterize the bacteriorhodopsin gene in Halobacterium halobium.
  • To determine the DNA sequence and regulatory elements of the bacteriorhodopsin gene.

Main Methods:

  • Isolation of a 5.3-kilobase restriction fragment containing the bacteriorhodopsin gene from Halobacterium halobium DNA.
  • DNA sequencing of the isolated fragment.
  • Use of a cloned cDNA fragment as a probe for gene identification.

Main Results:

Related Experiment Videos

  • The complete DNA sequence of the bacteriorhodopsin gene was determined (786 nucleotides for the structural gene).
  • The gene codes for a precursor protein with an N-terminal extension and a C-terminal aspartic acid.
  • The standard genetic code is used, with a preference for G or C at the third codon position; no intervening sequences or prokaryotic promoters were found.

Conclusions:

  • The bacteriorhodopsin gene in Halobacterium halobium is transcribed and translated using the standard genetic code.
  • The gene lacks introns and typical prokaryotic promoter sequences, suggesting unique regulatory mechanisms.
  • The mRNA structure includes a hairpin loop and a sequence complementary to 16S rRNA, potentially involved in translation initiation.