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Updated: Sep 30, 2026
Lethality Bioassay Using Artemia salina L.
Published on: October 11, 2022
Acute toxicity of TBT and IRGAROL in Artemia salina
Bakoulia Panagoula1, Marcouli Panayiota, Joan Iliopoulou-Georgudaki
1Department of Biology, Section of Animal Biology, Unit of Environmental Management Pollution and Ecotoxicology, University of Patras, Patras, Greece.
Abstract:
A 24-hour LC(50) bioassay method was carried out to study acute toxicity of Tributyltin (TBT) and IRGAROL (C(11)H(19)N(5)S) in Artemia salina. Five graded levels of both biocides were tested. According to the range test, the doses of TBT were 11.6 ng x l(-1), 21.0 ng x l(-1), 37.3 ng x l(-1), 65.2 ng x l(-1), and 116.5 ng x l(-1), and for IRGAROL were 1.0 mg x l(-1), 1.8 mg x l(-1), 3.2 mg x l(-1), 5.6 mg x l(-1), and 10 mg x l(-1). The determined LC(50) values were 41.41 ng x l(-1) and 1.62 mg x l(-1) respectively. These results indicate that in this system TBT is acutely more toxic than IRGAROL; however, both are proven environmentally toxic substances.
