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[RP-HPLC determination of adenosine in fermented Cordyceps]
1Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050.
Abstract
Objective:
A method for determination of adenosine in four fermented Cordyceps products by RP-HPLC is described.
Method:
C18 column was used with methanol-0.06 mol/L potassium phosphate monobasic-tetrahydrofuran (10:150:1.5) as mobile phase. The flow rate was 1 ml/min and detected at 260 nm.
Results:
The average recovery was 98.7% and relative standard deviation was 1.10% (n = 5).
Conclusion:
This method is simple, rapid and sensitive. The paper introduces the feasibility of supersonic extraction method taking the place of reflux method.