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Updated: Aug 21, 2026

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
Published on: June 30, 2019
Polymer-supported reagents for methylphosphorylation and phosphorylation of carbohydrates
1Department of Biomedical Sciences, College of Pharmacy, University of Rhode Island, 41 Lower College Road, Kingston 02881, USA. kpa0339u@postoffice.uri.edu
Abstract:
Two polymer-supported reagents for methylphosphorylation and phosphorylation of carbohydrates have been developed. p-Hydroxybenzyl alcohol and beta-mercaptoethanol were immobilized on cross-linked divinylbenzene-polystyrene copolymer and conjugated with methyl N,N-diisopropylchlorophosphoramidite. Carbohydrates were reacted with polymer-bound phosphitylating reagents. Further oxidation, with or without the methoxy group deprotection, and cleavage yielded methylphosphorylated or phosphorylated carbohydrates, respectively.
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