[Sporulation of anthrax bacilli]

Studies on Bac. antharcis sporulation were carried out. By modifying the medium of Kim et al., it was proven that 10 out of the 13 Bac. antharcis strains studied sporulated until the 72d h more than 99%. The remaining 3 strains sporulated more slowly. It was established that the nutrient medium could not ensure optimal sporulation conditions for all strains. The fact that the capsuleless vaccine "Ihtiman" sporulates 100% at the 24th to the 48th h is of considerable importance. The cultivation of this strain on synthetic medium does not change its immunogenic properties. No reversion to its primary state of virulence was observed.