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Continuous green biocatalytic processes using ionic liquids and supercritical carbon dioxide
Pedro Lozano1, Teresa de Diego, Daniel Carrié
1Departamento de Bioquímica y Biología Molecular B e Inmunología, Facultad de Química, Universidad de Murcia, P.O. Box 4021, E-30100 Murcia, España.
Chemical Communications (Cambridge, England)
|July 18, 2002
Summary
Candida antarctica lipase B in ionic liquids demonstrates high activity and stability in supercritical carbon dioxide for ester synthesis and chiral alcohol resolution via transesterification.
Area of Science:
- Biocatalysis and enzyme engineering
- Green chemistry and sustainable processes
- Supercritical fluid technology
Background:
- Enzyme immobilization is crucial for enhancing lipase stability and reusability.
- Ionic liquids offer unique solvent properties for biocatalytic applications.
- Supercritical carbon dioxide is an environmentally friendly reaction medium.
Purpose of the Study:
- To investigate the efficacy of soluble Candida antarctica lipase B (CALB) in ionic liquids.
- To evaluate the performance of CALB in supercritical carbon dioxide (scCO2).
- To assess the enzyme's utility in ester synthesis and kinetic resolution.
Main Methods:
- Immobilization of soluble CALB in specific ionic liquids.
- Performing transesterification reactions in supercritical carbon dioxide.
- Assessing synthetic activity, enantioselectivity, and operational stability.
Main Results:
- CALB in ionic liquids exhibited significant synthetic activity in scCO2.
- High enantioselectivity was observed in the kinetic resolution of 1-phenylethanol.
- The enzyme demonstrated good operational stability under scCO2 conditions.
- Successful butyl butyrate synthesis was achieved.
Conclusions:
- Ionic liquid-dissolved CALB is a robust biocatalyst for scCO2 applications.
- This system offers an efficient and green approach for ester synthesis and chiral resolution.
- Further exploration of enzyme-ionic liquid-scCO2 systems is warranted.