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Squalene-hopene cyclase: catalytic mechanism and substrate recognition
Tsutomu Hoshino1, Tsutomu Sato
1Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Ikarashi, Niigata 950-2181, Japan. hoshitsu@agr.niigata-u.ac.jp
Chemical Communications (Cambridge, England)
|July 18, 2002
Abstract:
Rapid progress on the catalytic mechanism and substrate recognition by squalene-hopene cyclase, which has occurred only in the last several years, is reported. A series of site-directed mutation experiments and some squalene analogues have provided deep insight into the polycyclization mechanism and catalytic sites in conjunction with the information from X-ray crystal data.