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[The detection of cyanobacterial cells by a non-fluorescent in situ hybridization method]
N L Bel'kova1, H M Chung, T S Ahn
1Limnological Institute, Siberian Division, Russian Academy of Sciences, ul. Ulan-Batorskaya 3, Irkutsk, 664033 Russia. belkova@lin.irk.ru
Mikrobiologiia
|July 26, 2002
Abstract:
Seven cyanobacterial strains (Anabaena macrospora NIER10016, Oscillatoria sp. NIER10042, Microcystis aeruginosa NIER10015, M. ichtyoblabe NIER10025 and NIER10040, M. novacekii NIER10029, and M. wesenbergii NIER10068) were tested by a non-fluorescent in situ hybridization method using two specific horseradish peroxidase--labeled oligonucleotide probes and two chromogenic substrates. This approach was shown to be appropriate for analysis of natural samples.