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Development and testing of a detection method for liquid chromatography based on aerosol charging
Roy W Dixon1, Dominic S Peterson
1Chemistry Department, California State University, Sacramento 95819-6057, USA. rdixon@csus.edu
Analytical Chemistry
|July 27, 2002
Summary
A novel aerosol charge detection method for High-Performance Liquid Chromatography (HPLC) was developed. This technique charges aerosol particles and measures current, offering sensitive detection for various compounds.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Aerosol-based detection methods for High-Performance Liquid Chromatography (HPLC) have been previously utilized.
- These methods typically convert HPLC effluent into an aerosol for optical detection.
Purpose of the Study:
- To introduce and evaluate a new aerosol-based detection method for HPLC, termed aerosol charge detection.
- To assess the performance of this novel detector using flow injection analysis and reversed-phase HPLC.
Main Methods:
- The new method involves generating an aerosol from HPLC effluent and detecting it by charging aerosol particles.
- A commercial electrical aerosol size analyzer was employed for aerosol detection.
- The detector was tested with flow injection analysis and reversed-phase HPLC using various analytes.
Main Results:
- Linear signal response was observed for sodium sulfate within the 0.2-100 µg mL⁻¹ concentration range.
- Minimum mass and concentration detection limits were determined to be 0.2 ng and 10 ng mL⁻¹, respectively.
- Similar linear response and detection limits were achieved for glucose using reversed-phase HPLC.
Conclusions:
- Aerosol charge detection offers a viable new method for HPLC analysis.
- The electrical aerosol size analyzer aids in improving the signal-to-noise ratio by removing small charged particles.
- This technique demonstrates sensitivity and linearity suitable for chromatographic applications.