Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Matrix-free methods for laser desorption/ionization mass spectrometry.

Dominic S Peterson1

  • 1Los Alamos National Laboratory, Chemistry Division, Mailstop K484, Los Alamos, New Mexico 87545, USA. DominicP@lanl.gov

Mass Spectrometry Reviews
|September 13, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Separation of actinides using capillary extraction chromatography-inductively coupled plasma mass spectrometry.

Journal of chromatographic science·2009
Same author

Evaluation of flow scintillation analysis for the determination of Sr-90 in bioassay samples.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2008
Same author

Optimization of extraction chromatography separations of trace levels of actinides with ICP-MS detection.

Journal of separation science·2007
Same author

Solid supports for micro analytical systems.

Lab on a chip·2005
Same author

Porous polymer monolith for surface-enhanced laser desorption/ionization time-of-flight mass spectrometry of small molecules.

Rapid communications in mass spectrometry : RCM·2004
Same author

Dual-function microanalytical device by in situ photolithographic grafting of porous polymer monolith: integrating solid-phase extraction and enzymatic digestion for peptide mass mapping.

Analytical chemistry·2004
Same journal

Mass Spectrometry Insights Into Post-Translational Modifications in Extracellular Vesicles.

Mass spectrometry reviews·2026
Same journal

Standardization Challenges and Breakthroughs in Apolipoprotein B Detection: From Immunoassays to LC-MS/MS.

Mass spectrometry reviews·2026
Same journal

The Rise of Trapped Ion Mobility Spectrometry: Principles, Applications, and Recent Developments.

Mass spectrometry reviews·2026
Same journal

Carlito B. Lebrilla: Building Instruments, Fields and Opportunities From the Ground up.

Mass spectrometry reviews·2026
Same journal

Recent Advances in Top-Down Proteomics for Single-Cell Research.

Mass spectrometry reviews·2026
Same journal

Applications of Top-Down Protein Analysis in Clinical Diagnostics.

Mass spectrometry reviews·2026
See all related articles

Matrix-free laser desorption/ionization mass spectrometry (LDI-MS) offers a solution for analyzing small molecules, overcoming limitations of traditional matrix-assisted laser desorption/ionization (MALDI). This review explores innovative matrix-free techniques for enhanced small molecule analysis.

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Spectroscopy

Background:

  • Matrix-assisted laser desorption/ionization (MALDI) is a powerful soft ionization technique for analyzing polymers and proteins.
  • A key limitation of MALDI is its difficulty in analyzing low molecular weight compounds (<1,000 m/z) due to matrix interference.
  • There is a growing need for effective methods to analyze small molecules using laser desorption/ionization mass spectrometry.

Purpose of the Study:

  • To review and examine matrix-free laser desorption/ionization (LDI) mass spectrometry approaches for small molecule analysis.
  • To define matrix-free LDI methods as those not requiring analyte-matrix co-crystallization.
  • To explore ionization mechanisms and applications of these advanced LDI techniques.

Main Methods:

Related Experiment Videos

  • Review of literature on matrix-free laser desorption/ionization (LDI) mass spectrometry.
  • Focus on techniques such as desorption/ionization on silicon (DIOS), sol-gels, and carbon-based microstructures.
  • Analysis of ionization mechanisms and practical applications in small molecule detection.

Main Results:

  • Matrix-free LDI techniques provide viable alternatives for analyzing low molecular weight compounds.
  • Methods like DIOS, sol-gels, and carbon microstructures avoid matrix-related interferences.
  • These approaches enable sensitive and selective analysis of small molecules.

Conclusions:

  • Matrix-free LDI mass spectrometry is a promising field for overcoming MALDI's limitations in small molecule analysis.
  • Continued development of these techniques will expand their applicability in various scientific domains.
  • The reviewed methods offer significant advantages for the sensitive detection of small molecules.