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Reverse Oxygen Effect on gamma-Irradiation Induced Single Strand Breaks of Plasmid DNA
Chun-Lin Shao1, Masahiro Saito, Zeng-Liang Yu
1Institute of Plasma Physics, the Chinese Academy of Sciences, P P Box 1126, Hefei 230031, China. CLShao@ipncl.hfcas.ac.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|July 27, 2002
Abstract:
Oxygen effect on gamma-irradiation induced single strand breaks (SSB) of plasmid DNA was measured by the method of gel scanning. It was found that, in the presence of mannitol, the yield of SSB in N(2)-saturated DNA solution was larger than that in air-saturated solution so that the oxygen enhancement ratio (OER) of SSB became smaller than 1, which indicates the appearance of reverse oxygen effect of DNA SSB. At the same time, G(SSB) and its OER decreased with increasing concentration of mannitol.