Related Experiment Video
Updated: Jul 12, 2026
11:42
Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
Published on: October 10, 2014
[Transgenic animals bioreactors]
Ke-Mian Gou1, Xiao-Rong An, Jian-Hui Tian
1National Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China. goukm@mail.cau.edu.cn
Summary
Transgenic farm animals efficiently produce valuable human proteins in milk. Nuclear transfer and site-specific DNA recombination enhance production efficiency and speed, revolutionizing biopharmaceutical development.
Area of Science:
- Biotechnology
- Genetics
- Molecular Biology
Context:
- Transgenic farm animals offer a cost-effective method for producing complex human recombinant proteins.
- Traditional production methods face limitations in efficiency and quantity for certain proteins.
Purpose:
- To review current advancements in using transgenic animals as bioreactors for protein production.
- To highlight novel strategies like nuclear transfer and site-specific recombination.
Summary:
- This review covers gene selection, vector design, transgenic techniques, economic factors, and the potential of transgenic animals.
- Recent breakthroughs include nuclear transfer for improved efficiency, consistency, and faster development.
- Cre/loxP site-specific recombination systems offer new possibilities for generating advanced transgenic animals.
Impact:
- Accelerates the development of biopharmaceuticals and valuable commercial proteins.
- Enhances the efficiency and consistency of producing therapeutic proteins.
- Paves the way for novel applications in biotechnology and medicine through advanced genetic engineering.
Related Concept Videos
00:53Transgenic Organisms
Overview
00:53Transgenic Organisms
Overview
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
01:29Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
01:22Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
01:27Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...