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Erythropoietin modulates calcium influx through TRPC2
Xin Chu1, Joseph Y Cheung, Dwayne L Barber
1Henry Hood Research Program, The Sigfried and Janet Weis Center for Research, Geisinger Clinic, 100 N. Academy Avenue, Danville, PA 17822, USA.
The Journal of Biological Chemistry
|August 9, 2002
Summary
Erythropoietin (Epo) stimulates calcium entry in erythroid cells through TRPC2 channels. This study identifies TRPC2 as a key mediator of Epo-induced calcium influx in these cells.
Area of Science:
- Cell Biology
- Ion Channels
- Hematology
Background:
- Mammalian TRPC channels regulate calcium entry in nonexcitable cells.
- Erythropoietin (Epo) induces intracellular calcium ([Ca](i)) rise in erythroid cells via calcium channels.
Purpose of the Study:
- To investigate the role of murine TRPC channels in Epo-modulated calcium influx.
- To identify the specific TRPC isoform involved in Epo signaling in erythroid cells.
Main Methods:
- RT-PCR, Western blotting, and immunolocalization to detect TRPC expression.
- Co-transfection of Epo-R and TRPC isoforms in CHO cells.
- Measurement of intracellular calcium ([Ca](i)) changes using fluorescent dyes and digital imaging.
Main Results:
- TRPC2 showed high expression in Epo-dependent erythroid cell lines and primary erythroblasts.
- Epo stimulation of cells expressing Epo-R and TRPC2 significantly increased [Ca](i) compared to controls.
- Epo-induced calcium influx through TRPC2 required extracellular calcium.
Conclusions:
- Murine TRPC2 is a calcium-permeable channel expressed in erythroid cells.
- Erythropoietin modulates calcium influx through TRPC2 channels.