Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transgenic strategy for demonstrating nuclear reprogramming in the mouse.

Megan Munsie1, Carmel O'brien, Peter Mountford

  • 1Centre for Early Human Development, Monash Institute of Reproduction and Development, Monash Universitym Clayton, Victoria, Australia.

Cloning and Stem Cells
|August 16, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human stem cell-based embryo models: innovation, ethics, and policy.

Human reproduction (Oxford, England)·2026
Same author

Diversity-in-a-dish: A practical framework for hiPSC model development.

Stem cell reports·2026
Same author

Picking Up the Phone: Clinical Perspectives on Recontacting Patients and Their Families.

Journal of paediatrics and child health·2026
Same author

Stem cells in your YouTube feed: The complex landscape and unexpected mediators of online information.

Stem cell reports·2025
Same author

Who am I to take hope away? - A qualitative study of clinicians' experiences with patient and family enquiries about unproven therapies.

Patient education and counseling·2025
Same author

Emerging Ethical Challenges in Manufacturing of Cell-Based Medicines.

Advances in experimental medicine and biology·2025
Same journal

Preimplantation development of somatic cell cloned embryos in the common marmoset (Callithrix jacchus).

Cloning and stem cells·2009
Same journal

Identification of multipotent cytotrophoblast cells from human first trimester chorionic villi.

Cloning and stem cells·2009
Same journal

Enhanced generation of myeloid lineages in hematopoietic differentiation from embryonic stem cells by silencing transcriptional repressor Twist-2.

Cloning and stem cells·2009
Same journal

High-efficiency production of subculturable vascular endothelial cells from feeder-free human embryonic stem cells without cell-sorting technique.

Cloning and stem cells·2009
Same journal

Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells.

Cloning and stem cells·2009
Same journal

Pluripotency and reprogramming: meeting report on the Fifth International Meeting of the Stem Cell Network North Rhine Westphalia.

Cloning and stem cells·2009
See all related articles

Nuclear reprogramming in cloned embryos was monitored using lacZ reporter genes. Successful reactivation of these genes in somatic cell nuclear transfer (SCNT) embryos indicates successful reprogramming, potentially improving cloning efficiency.

Area of Science:

  • Developmental Biology
  • Reproductive Biology
  • Genetics

Background:

  • Somatic cell nuclear transfer (SCNT) can restore totipotency to differentiated cell nuclei.
  • Nuclear reprogramming requires significant changes in gene expression from somatic to embryonic patterns.
  • Monitoring reprogramming is crucial for improving SCNT efficiency.

Purpose of the Study:

  • To demonstrate nuclear reprogramming in SCNT embryos using developmentally regulated lacZ reporter genes.
  • To assess the reactivation of specific genes (Oct4, COB54) as indicators of successful reprogramming.
  • To explore the potential of reporter gene strategies for selecting reprogrammed embryos.

Main Methods:

  • Generation of SCNT embryos by transferring adult cumulus cell nuclei into enucleated oocytes.

Related Experiment Videos

  • Utilization of transgenic mice with lacZ reporter genes linked to Oct4 or COB54.
  • Assessment of lacZ reporter gene expression via X-gal staining in SCNT embryos.

    • Main Results:

    • Reactivation of lacZ reporter genes was observed in SCNT embryos, indicating nuclear reprogramming.
    • Gene reactivation timing and coordination were consistent with control embryos.
    • Reduced or absent reporter gene expression in some SCNT embryos suggested reprogramming failure.

    Conclusions:

    • Developmentally regulated reporter genes, like lacZ, can effectively monitor nuclear reprogramming post-SCNT.
    • This approach offers a potential strategy for identifying and selecting reprogrammed embryos to enhance cloning efficiency.
    • Further development with real-time reporters (e.g., GFP) could improve monitoring and selection.