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Updated: Sep 29, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Detection of the HBV DNA with Sybr green 1 realtime polymerase chain reaction]
Yan Wang1, Xiaoyuan Xu, Wei He
1Department of Infectious Diseases, First Hospital of Beijing University, Beijing 100034, China.
Background:
To search a novel sensitive, specific and lower cost method applicable for quantitative analysis of the hepatitis B virus DNA extensively.
Methods:
Quantitative analysis of the DNA from 100 sera by real-time PCR with Sybr green 1. The results of Sybr's assay were compared with the results obtained with Taqman's fluorescent quantitative assay.
Results:
Taqman real-time PCR could help evaluate the level of virus reliably. The results of Sybr's assay were in agreement with the Taqman's assay, but detection rate was lower.
Conclusions:
Sybr green 1 real-time PCR appeared to be convenient and cheap, but detection rate was lower.

