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Production of long-chain levan by a sacC insertional mutant from Bacillus subtilis 327UH
Toshio Shida1, Kohsuke Mukaijo, Shu Ishikawa
1Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
Bioscience, Biotechnology, and Biochemistry
|September 13, 2002
Abstract:
A hyper extracellular protein producer, Bacillus subtilis 327UH, produced large amounts of levan in a medium containing 20% sucrose, and the yield of levan after 10 hours was more than 60%, when based on the fructose amount of sucrose. After transformation of 327UH with a levanase-deficient 168SC (sacC::Cm(r)) chromosomal DNA, a Cm(r) transformant 327UHSC (sacC::Cm(r) degSU(Hy)) produced 3 times longer levan than that of the wild type.