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DNA binding properties of oligodeoxynucleotides containing pyrrolidino C-nucleosides
Adrian Häberli1, Christian J Leumann
1Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, Switzerland.
Organic Letters
|September 14, 2002
Abstract:
We have incorporated pyrrolidino-C-nucleosides (pyrrolidino-pseudonucleosides) containing the base uracil and N-1-methyl uracil into oligodeoxynucleotides and compared their thermal duplex and triplex stabilities with unmodified or pseudouridine-containing oligodeoxynucleotides. We find relative destabilizations of triplex formation by ca. -13 to -1 degrees C per modification (relative to thymidine) in a strongly sequence dependent mode. Duplex formation is less destabilizing and more homogeneous with -4 to -2 degrees C per modification.