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Related Experiment Videos

Cu2+ Reduction by Tomato Root Plasma Membrane Vesicles.

M. J. Holden1, T. J. Crimmins, R. L. Chaney

  • 1Department of Biological Sciences, The George Washington University, Washington, DC 20052 (M.J.H., T.J.C.).

Plant Physiology
|July 1, 1995
PubMed
Summary
This summary is machine-generated.

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Tomato roots reduce copper (Cu2+) via plasma membrane vesicles, particularly when iron-deficient. However, this reduction likely has minimal physiological relevance in typical soil conditions.

Area of Science:

  • Plant Physiology
  • Biochemistry
  • Environmental Science

Background:

  • Copper (Cu) is an essential micronutrient for plants, but its availability in soil can be limited.
  • Plasma membrane-bound reductases in plant roots play roles in nutrient acquisition and detoxification.
  • Iron (Fe) deficiency can alter root plasma membrane activities, potentially impacting other metal reductions.

Purpose of the Study:

  • To investigate the reduction of Cu2+ by plasma membrane vesicles from tomato (Lycopersicon esculentum Mill.) roots.
  • To determine the role of Fe deficiency in Cu reduction activity.
  • To identify the specific reductases involved in Cu reduction and assess its physiological relevance.

Main Methods:

  • Isolation of plasma membrane vesicles from tomato roots grown in hydroponic culture.

Related Experiment Videos

  • Measurement of Cu, Fe, and ferricyanide reduction by plasma membrane vesicles.
  • Solubilization and chromatographic separation of plasma membrane electron transport systems.
  • Assay of Cu chelate reduction using the Geochem PC program to determine Cu activity.

    • Main Results:

    • Plasma membrane preparations from Fe-deficient plants showed increased activity in pyridine-nucleotide-dependent reductions compared to Fe-sufficient plants.
    • Fe-chelate reductase was identified as the likely enzyme responsible for Cu reduction.
    • The rate of Cu reduction correlated with Cu activity, suggesting free Cu2+ is the substrate.
    • Significant Cu reduction was only observed under conditions mimicking Cu-enriched or toxic soils.

    Conclusions:

    • Tomato root plasma membranes possess the capacity to reduce Cu2+.
    • Fe deficiency enhances the activity of reductases involved in Cu reduction.
    • The physiological relevance of this Cu reduction mechanism in tomato roots under normal soil conditions is likely minimal.