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Fructosamine assay using albumin extracted from serum.
Emi Ohkawara1, Yukio Nohara, Yoshihiko Kanno
1Faculty of Pharmaceutical Sciences, Teikyo University, Tsukui-gun, Kanagawa, Japan. emi_rnr-1968@mtj.biglobe.ne.jp
Biological & Pharmaceutical Bulletin
|September 17, 2002
Summary
This study developed a new method for measuring albumin fructosamine in serum. The improved assay offers a more accurate reflection of blood glucose control compared to traditional methods.
Area of Science:
- Clinical Chemistry
- Biochemistry
Background:
- Serum fructosamine is a marker for glycemic control.
- Existing assays can be affected by interfering substances and protein variations.
Purpose of the Study:
- To develop and validate a novel fructosamine assay using purified albumin.
- To improve the accuracy of fructosamine measurements for assessing glycemic control.
Main Methods:
- Albumin was extracted from serum using trichloroacetic acid and ethanol.
- A nitroblue tetrazolium-based assay was employed with 1-deoxy-1-p-toluidino-D-fructose (DTF) as a calibrator.
- Albumin fructosamine was quantified and corrected for albumin concentration.
Main Results:
- The assay demonstrated no interference from low molecular weight substances or other proteins.
- Corrected albumin fructosamine values showed a stronger correlation with fasting blood glucose (r=0.735) than standard serum fructosamine (r=0.514).
Conclusions:
- The novel albumin fructosamine assay provides a more reliable measure of long-term glycemic control.
- This method overcomes limitations of existing assays, offering enhanced diagnostic accuracy.