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[Localization of Listeria in white mice]
Abstract:
A total of 115 albino mice were infected through venous, subcutaneous, and oral administration of a suspension of 32P-labeled Listeria organisms at the concentration of 1.5 billion microbial cells per cu. cm. Each day following inoculation as many as four animals on an average were investigated (up to 120th hour) for all of the routes of infection used. It was established that via venous application the Listeria organisms reached all viscera and the brain in highest numbers. The greatest localization of Listeria introduced venously and orally was found in the first 24 hours in the liver. The localization of the organisms in animals infected per os proved similar to that found with the venous route of inoculation, however, showing weaker intensity. Listeria were found in the brain regardless of the route of infection.
Insights
This study tracked 32P-labeled Listeria monocytogenes in mice after intravenous, subcutaneous, and oral infection. Intravenous Listeria spread most widely, reaching viscera and brain, with peak liver localization within 24 hours.
Area of Science:
- Microbiology
- Immunology
- Infectious Diseases
Context:
- Investigating the dissemination patterns of bacterial pathogens is crucial for understanding infection dynamics.
- Listeria monocytogenes is an important foodborne pathogen with significant public health implications.
- Radioisotope labeling (32P) allows for quantitative tracking of microbial distribution in vivo.
Purpose:
- To determine the biodistribution and localization of Listeria monocytogenes following different routes of administration in a murine model.
- To compare the efficiency of Listeria monocytogenes spread via venous, subcutaneous, and oral inoculation.
- To identify target organs and the temporal dynamics of Listeria monocytogenes infection.
Summary:
- Albino mice (n=115) were infected with 32P-labeled Listeria monocytogenes (1.5 x 10^9 cells/cm³) via intravenous, subcutaneous, and oral routes.
- Organism distribution was assessed daily up to 120 hours post-inoculation.
- Intravenous administration resulted in the highest Listeria concentrations in all viscera and the brain.
- Peak Listeria localization in the liver occurred within 24 hours for both venous and oral routes.
- Oral infection showed similar, albeit less intense, distribution patterns compared to the intravenous route.
- Listeria monocytogenes was detected in the brain irrespective of the inoculation route.
Impact:
- Provides critical data on Listeria monocytogenes pathogenesis and host-pathogen interactions.
- Informs the development of targeted therapeutic and prophylactic strategies against Listeria infections.
- Highlights the importance of the intravenous route for rapid and widespread dissemination, including to the central nervous system.