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Ratiometric probes for hydrogencarbonate analysis in intracellular or extracellular environments using europium
Yann Bretonnière1, Martin J Cann, David Parker
1Department of Chemistry, University of Durham, South Road, Durham, UK DH1 3LE.
Chemical Communications (Cambridge, England)
|September 26, 2002
Abstract:
A series of six, cationic, zwitterionic and anionic Eu complexes has been examined for the analysis of hydrogencarbonate concentration in the intracellular and extracellular ranges; an anionic complex incorporating three glutarate residues and a sensitising acridone chromophore (lambda exc = 410 nm) exhibits a 69% change in the intensity ratio of the 618/588 nm Eu emission bands between 5 and 15 mM HCO3- in a cell lysate medium.