Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spectral imaging fluorescence microscopy.

Tokuko Haraguchi1, Takeshi Shimi, Takako Koujin

  • 1CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan.

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
|September 26, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interaction Between Rec8 and Mis4 Is Required for Axis-Loop Chromatin Formation and Homologous Chromosome Recombination During Meiosis.

Genes to cells : devoted to molecular & cellular mechanisms·2026
Same author

OptoChaperone─A Biohybrid Tool for Regulating Protein Condensates in Cells and In Vitro.

Journal of the American Chemical Society·2026
Same author

Phase-based computational adaptive optics enables artifact-free super-resolution microscopy.

Communications engineering·2026
Same author

GSH as an A-A Type Allosteric Activator of PKM2: Modulating Cancer Cell Homeostasis and Ferroptosis Susceptibility.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Probing Nanomechanics by Direct Indentation Using Nanoendoscopy-AFM Reveals the Nuclear Elasticity Transition in Cancer Cells.

ACS applied nano materials·2025
Same author

Use of Nucleoporin-Conjugated Beads to Study the Nuclear Pore Complex Assembly on the Nuclear Membrane.

Methods in molecular biology (Clifton, N.J.)·2025

This study introduces a spectral imaging fluorescence microscope for enhanced cellular analysis. It enables precise detection of spectral shifts in fluorescent dyes and differentiation of overlapping signals, advancing live-cell imaging techniques.

Area of Science:

  • Cellular and Molecular Imaging
  • Biophysics
  • Spectroscopy

Background:

  • Fluorescence microscopy is crucial for visualizing cellular processes.
  • Distinguishing between spectrally similar fluorophores in live cells presents a significant challenge.
  • Existing methods often lack the spectral resolution needed for complex biological samples.

Purpose of the Study:

  • To develop and demonstrate a spectral imaging fluorescence microscope for enhanced resolution in live-cell imaging.
  • To showcase the system's capability in detecting spectral changes in fluorescent dyes, such as those involved in FRET.
  • To resolve overlapping emission spectra from common fluorophores like FITC and GFP.

Main Methods:

  • Utilized a confocal laser scanning microscope integrated with grating optics for spectral resolution.

Related Experiment Videos

  • Employed spectral imaging techniques to capture detailed emission spectra from fluorescently labeled live cells.
  • Applied the system to analyze spectral shifts and differentiate spectrally similar fluorescent signals.

    • Main Results:

    • Achieved high spectral and temporal resolution in fluorescence microscopy of living cells.
    • Successfully detected spectral changes associated with fluorescence resonance energy transfer (FRET).
    • Demonstrated the ability to resolve and distinguish between largely overlapping spectra of fluorescein isothiocyanate (FITC) and green fluorescent protein (GFP).

    Conclusions:

    • The developed spectral imaging fluorescence microscope significantly enhances the capabilities of live-cell analysis.
    • This technology provides a powerful tool for studying molecular interactions and dynamics using FRET.
    • It offers improved differentiation of fluorophores, enabling more complex multiplexed imaging in biological research.