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Applications of pulsed ultrafiltration-mass spectrometry
Benjamin M Johnson1, Dejan Nikolic, Richard B van Breemen
1Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, Illinois 60612-7231,USA.
Mass Spectrometry Reviews
|October 10, 2002
Summary
Pulsed ultrafiltration-mass spectrometry (PUF-MS) enables rapid discovery of biologically active molecules. This method screens libraries and natural products, investigates ligand-receptor binding, and identifies drug metabolites, offering high throughput and sensitivity.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Pharmacology
Background:
- Pulsed ultrafiltration-mass spectrometry (PUF-MS) is a versatile analytical technique.
- It is crucial for identifying and characterizing biologically active small molecules.
Purpose of the Study:
- To highlight the diverse applications of PUF-MS in drug discovery and development.
- To showcase its utility in screening, binding studies, and metabolic analysis.
Main Methods:
- Utilizes pulsed ultrafiltration coupled with mass spectrometry for sensitive detection.
- Employs solution-phase ligand-screening assays for receptor-ligand studies.
- Integrates with LC-MS-MS for metabolic screening and toxicity prediction.
Main Results:
- PUF-MS facilitates screening of combinatorial libraries and natural product extracts.
- Enables rapid determination of ligand-receptor binding parameters.
- Identifies cytochrome P450 substrates and predicts potential toxicity of metabolites.
Conclusions:
- PUF-MS offers high sensitivity and selectivity for analyzing complex mixtures.
- Applicable to diverse areas including "reverse pharmacology" and metabolite identification.
- Accelerates the discovery and development of novel therapeutic compounds.