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Updated: Aug 8, 2026
Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
Published on: July 28, 2009
Preparation and identification of monoclonal antibodies against human brain-derived neurotrophic factor
Jin Su1, Ya-Li Zhang, Chang-Xuan You
1Department of Respiratory Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To prepare monoclonal antibodies (mAbs) against human brain-derived neurotrophic factor (BDNF).
Methods:
BALB/C mice were immunized with purified BDNF protein in conjunction with acid-treated Salmonella (antigen:thallus=1:5), and mAbs were subsequently derived by hybridoma technique.
Results:
Three hybridoma cell lines secreting anti-BDNF mAbs were obtained, designated as B1, B2 and 4D1 respectively and all categorized into IgG1 subtype. The titers of the mAbs in the ascitic fluid of the rats ranged from 1x10(6) to 1x10(5), and their relative affinities were B2>B1>4D1.
Conclusion:
mAbs against BDNF are successfully prepared, which can be instrumental for further study of the expression and distribution of BDNF in vivo, and the detection of BDNF produced by genetic engineering.
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