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Rapid HPLC measurement of buflomedil in plasma in poisoning cases
I Forfar-Bares1, F Pehourcq, C Jarry
1EA2962-Pharmacochimie, Université Victor Segalen Bordeaux 2, Bordeaux, France. isabelle.bares@chimphys.u-bordeaux2.fr
Abstract:
A rapid high-performance liquid chromatographic method for the determination of buflomedil in human plasma is described. It requires a single liquid-liquid extraction step from 1 mL of plasma with diethyl ether followed by chromatography on a Nova Pak C(18) reversed-phase column and detection by ultaviolet light. Metoclopramide was used as internal standard. The method is sensitive with a quantification limit at 500 ng/mL. It was used for the determination of buflomedil in biological fluids in poisoning cases.
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