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Related Experiment Videos

Profiling pH changes in the electrospray plume.

Shaolian Zhou1, Benjamin S Prebyl, Kelsey D Cook

  • 1Department of Chemistry, University of Tennessee, Knoxville 37996-1600, USA.

Analytical Chemistry
|October 17, 2002
PubMed
Summary
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Researchers used laser-induced fluorescence spectrometry to track pH changes in evaporating electrospray droplets. Acidic droplets showed greater pH decrease than expected, possibly due to cooling or charge effects.

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Electrospray ionization (ESI) is a crucial technique in mass spectrometry.
  • Understanding droplet behavior during ESI is vital for accurate analysis.

Purpose of the Study:

  • To investigate pH variations in evaporating electrospray droplets.
  • To correlate pH changes with initial droplet conditions and ion modes.

Main Methods:

  • Utilized laser-induced fluorescence spectrometry.
  • Employed carboxy SNARF-1, a pH-sensitive fluorescent dye.
  • Monitored emission spectra during droplet evaporation in an electrospray plume.

Main Results:

  • Observed pH changes were dependent on initial droplet pH and polarity.

Related Experiment Videos

  • Initially acidic droplets exhibited a pH decrease along the spray axis in both positive and negative ion modes.
  • The extent of pH decrease exceeded predictions based solely on solvent evaporation.

    • Conclusions:

    • The observed pH changes suggest phenomena beyond simple solvent evaporation.
    • Potential contributing factors include droplet cooling, subdivision, or heterogeneous charge distribution within the spray.