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Serological differentiation of infections with dengue virus serotypes 1 to 4 by using recombinant antigens
Diana Ludolfs1, Stefan Schilling, Jan Altenschmidt
1Bernhard-Nocht Institute for Tropical Medicine, Hamburg, Germany.
Journal of Clinical Microbiology
|November 1, 2002
Abstract:
The B domains of dengue virus serotypes 1 to 4 were expressed in Escherichia coli. The purified proteins were applied to immunoblot strips to detect serotype-specific antibodies in paired serum samples from 41 patients with primary and secondary dengue infections. A close correlation between the results obtained with the immunoblot strips and by type-specific reverse transcription-PCR (T. Laue, P. Emmerich, and H. Schmitz, J. Clin. Microbiol. 37:2543-2547, 1999) was observed.