Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The centrosome is a dynamic structure that ejects PCM flares.

Timothy L Megraw1, Sandhya Kilaru, F Rudolf Turner

  • 1Department of Biology, Indiana University, Bloomington, IN 47405, USA.

Journal of Cell Science
|November 5, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conserved Molecular Responses to Arsenite Exposure in <i>Drosophila melanogaster</i>.

bioRxiv : the preprint server for biology·2026
Same author

Alternative Vertebrate and Invertebrate Model Organisms Show Similar Sensitivity as Rodents to a Diverse Set of Chemicals.

Environmental science & technology·2025
Same author

A distinct isoform of Msp300 (nesprin) organizes the perinuclear microtubule-organizing center in adipocytes.

Molecular biology of the cell·2025
Same author

Regulation of misfolded protein aggregation and degradation by SUMOylation in budding yeast.

Molecular biology of the cell·2025
Same author

Zika virus NS3 drives the assembly of a viroplasm-like structure.

bioRxiv : the preprint server for biology·2024
Same author

Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization.

Molecular biology of the cell·2024
Same journal

Potential role of myeloid bodies in protection against photo-oxidative damage of the retinal pigment epithelium.

Journal of cell science·2026
Same journal

From the FAZ to the TAC: Widespread cytoskeletal engagement of T. brucei KMP11.

Journal of cell science·2026
Same journal

Branched actin segregates endocytic cargo to control sorting and fission.

Journal of cell science·2026
Same journal

FAM122A inhibition of PP2A-B55 through a bipartite binding mechanism.

Journal of cell science·2026
Same journal

Disentangling the response to lysosomal damage.

Journal of cell science·2026
Same journal

The force, form and function of the nucleus.

Journal of cell science·2026
See all related articles

Centrosomin (Cnn) protein forms dynamic

Area of Science:

  • Cell Biology
  • Developmental Biology

Background:

  • Centrosomes are critical microtubule-organizing centers in eukaryotic cells.
  • The Centrosomin (Cnn) protein is a core component of Drosophila melanogaster centrosomes, essential for early embryonic development.

Purpose of the Study:

  • To investigate the dynamic behavior of Cnn protein at centrosomes in live Drosophila embryos.
  • To characterize the nature and regulation of Cnn-containing extracentrosomal particles, termed 'flares'.

Main Methods:

  • Expression of a functional Cnn-GFP fusion protein in Drosophila embryos.
  • Live imaging of GFP-Cnn localization and dynamics.
  • Immunofluorescent staining with anti-Cnn antibodies.
  • Disruption of microtubule and microfilament arrays.

Related Experiment Videos

  • Injection of rhodamine-actin to visualize the actin cage.

    • Main Results:

    • GFP-Cnn localization mirrors endogenous Cnn patterns and rescues cnn mutant lethality.
    • Extracentrosomal Cnn particles, named 'flares', exhibit dynamic radial movement dependent on microtubules.
    • Flare activity is cell-cycle dependent, peaking during telophase and interphase.
    • Flares are associated with D-TACC and are contained within the actin cage.
    • Microfilament disruption enhances flare movement, suggesting an interaction with the actin cytoskeleton.

    Conclusions:

    • Drosophila centrosomes actively eject Cnn-containing pericentriolar material particles ('flares').
    • Flare movement is mediated by dynamic astral microtubules and influenced by the cell cycle.
    • Flares may play a role in organizing the actin cytoskeleton during syncytial cleavage.