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Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
Published on: March 17, 2014
Molecular control of ciliary neuron development: BMPs and downstream transcriptional control in the parasympathetic
1Max-Planck-Institut für Hirnforschung, Abteilung Neurochemie, Deutschordenstrasse 46, 60528 Frankfurt/Main, Germany.
Abstract:
The generation of noradrenergic sympathetic neurons is controlled by BMPs and the downstream transcription factors Mash1, Phox2b, Phox2a and dHand. We examined the role of these signals in developing cholinergic parasympathetic neurons. The expression of Mash1 (Cash1), Phox2b and Phox2a in the chick ciliary ganglion is followed by the sequential expression of panneuronal, noradrenergic and cholinergic marker genes. BMPs are expressed at the site where ciliary ganglia form and are essential and sufficient for ciliary neuron development. Unlike sympathetic neurons, ciliary neurons do not express dHand; noradrenergic gene expression is eventually lost but can be maintained by ectopic dHand expression. Together, these results demonstrate a common BMP dependence of sympathetic neurons and parasympathetic ciliary neurons and implicate dHand in the maintenance of noradrenergic gene expression in the autonomic nervous system.
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