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cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo
Ichiro Yamamoto1, Akira Tsukada, Noboru Saito
1Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan.
Biochimica Et Biophysica Acta
|November 13, 2002
Abstract:
We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.