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Age-related changes in inducible osteogenic precursors
Yu F Gorskaya1, E Yu Shuklina, V G Nesterenko
1Laboratory of Cell Immunity, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow.
Bulletin of Experimental Biology and Medicine
|November 16, 2002
Summary
Aging increases urinary bladder osteoinductive activity, but decreases osteogenic precursor cells. Urinary bladder transitional epithelium growth factors are crucial for stromal precursor proliferation in the spleen.
Area of Science:
- Urology
- Gerontology
- Cell Biology
Background:
- The urinary bladder's transitional epithelium possesses osteoinductive properties.
- Age-related changes in bone metabolism and precursor cell populations are not fully understood.
Purpose of the Study:
- To investigate the impact of aging on the osteoinductive activity of the urinary bladder epithelium.
- To examine age-related changes in osteogenic precursor cells and their responsiveness to urinary bladder osteoinductive factor.
- To determine the role of urinary bladder transitional epithelium-derived growth factors in stromal precursor proliferation.
Main Methods:
- Comparative analysis of osteoinductive activity in aging guinea pig urinary bladders.
- Quantification of inducible osteogenic precursors and assessment of their sensitivity to urinary bladder osteoinductive factor.
- In vitro experiments to evaluate the requirement of urinary bladder transitional epithelium growth factors for splenic stromal precursor proliferation.
Main Results:
- Osteoinductive activity in the aging guinea pig urinary bladder transitional epithelium increased over threefold.
- The number of inducible osteogenic precursor cells and their sensitivity to urinary bladder osteoinductive factor significantly decreased with age.
- Proliferation of specific splenic stromal precursors was found to depend on both platelet-derived factors and growth factors from the urinary bladder transitional epithelium.
Conclusions:
- Aging significantly alters the osteoinductive capacity of the urinary bladder epithelium, increasing its activity while diminishing precursor cell function.
- The urinary bladder transitional epithelium produces essential growth factors that support the proliferation of stromal precursors in extramedullary sites like the spleen.
- These findings highlight a novel role for the urinary bladder in regulating extramedullary hematopoiesis and bone metabolism, particularly in the context of aging.