Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimizing vaccine design for cellular processing, MHC binding and TCR recognition.

A Sette1, M Newman, B Livingston

  • 1Epimmune Inc., San Diego, CA 92121, USA.

Tissue Antigens
|November 26, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prediction of preterm birth by measurement of cervical length on transvaginal ultrasound and magnetic resonance imaging in pregnancies complicated by twin-twin transfusion syndrome and treated with laser surgery.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2023
Same author

Immune signatures underlying post-acute COVID-19 lung sequelae.

Science immunology·2021
Same author

Pathways to post-traumatic growth in cancer patients: moderated mediation and single mediation analyses with resilience, personality, and coping strategies.

Journal of affective disorders·2020
Same author

An effective CTL peptide vaccine for Ebola Zaire Based on Survivors' CD8+ targeting of a particular nucleocapsid protein epitope with potential implications for COVID-19 vaccine design.

Vaccine·2020
Same author

Cripto-1 vaccination elicits protective immunity against metastatic melanoma.

Oncoimmunology·2016
Same author

Sequence conservation predicts T cell reactivity against ragweed allergens.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2016
Same journal

Corrigendum.

Tissue antigens·2015
Same journal

Nomenclature for factors of the HLA system, update September 2015.

Tissue antigens·2015
Same journal

Nomenclature for factors of the HLA system, update August 2015.

Tissue antigens·2015
Same journal

Nomenclature for factors of the HLA system, update July 2015.

Tissue antigens·2015
Same journal

Four amino acid exchanges located in the alpha-2 domain specify the novel HLA-B*50:20 allele.

Tissue antigens·2015
Same journal

A novel HLA-A*02 variant, HLA-A*02:575, detected in a Taiwanese bone marrow hematopoietic stem cell donor.

Tissue antigens·2015
See all related articles

This review details methods for designing and testing multiepitope vaccines against infectious diseases and cancer. We optimized vaccine processing and evaluated human leukocyte antigen (HLA) binding and T-cell receptor recognition for improved efficacy.

Area of Science:

  • Vaccinology
  • Immunology
  • Bioengineering

Background:

  • Multiepitope vaccines offer a promising strategy for targeting complex diseases like cancer and infectious diseases.
  • Designing effective multiepitope vaccines requires careful consideration of epitope selection, processing, and presentation.
  • Human Leukocyte Antigen (HLA) restriction plays a critical role in vaccine immunogenicity.

Purpose of the Study:

  • To describe methods for testing and designing multiepitope vaccines.
  • To optimize the processing and immunogenicity of vaccine constructs.
  • To evaluate strategies for enhancing HLA binding affinity, patient coverage, and T-cell receptor recognition.

Main Methods:

  • In vitro antigenicity assays to test vaccine performance.

Related Experiment Videos

  • In vivo immunogenicity assays using HLA-transgenic models.
  • Design of spacer residues to optimize epitope processing for both MHC class I and class II presentation.
  • Assessment of HLA binding affinity and T-cell receptor recognition.

    • Main Results:

    • Developed and refined methods for multiepitope vaccine design and testing.
    • Demonstrated optimization of vaccine processing through strategic use of spacer residues.
    • Established assays to evaluate key parameters of vaccine efficacy, including HLA binding and T-cell recognition.

    Conclusions:

    • The described methods provide a framework for developing robust multiepitope vaccines.
    • Optimization of epitope processing and presentation is crucial for vaccine effectiveness.
    • Comprehensive testing strategies are essential for predicting and improving clinical outcomes of multiepitope vaccines.